Detection of Antibody Display Phage without Clearing of Bacterial Culture
نویسندگان
چکیده
منابع مشابه
Detection of antibody display phage without clearing of bacterial culture.
Filamentous phage display has become a powerful method for the discovery of affinity-binding reagents (6). Linear and constrained peptides, antibody fragments (scFvs and Fabs) and some alternative binding domains have all been displayed on phage particles by fusing to one of the phage coat proteins. Although several phage proteins (derived from gVIII, gVI, gVII and gIX) have all been used as fu...
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Vibrio parahaemolyticus is a halophilic Gramnegative facultative anaerobe commonly found in estuarine waters and in seafood like shellfish. It has been implicated in outbreaks of gastro-enteritis in several countries [1]. Food borne illness caused by V. parahaemolyticus results chiefly from the consumption of insufficiently heated or raw seafood, especially in the summer months. The standard me...
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The selection of antibodies from combinatorial libraries displayed on the surface of filamentous phage has become an important methodology for the generation of reagent, diagnostic, and therapeutic molecules and for the study of natural immune responses. Using this technique, antibody genes have been cloned from multiple species or expressed directly from large man-made repertoires of antibody-...
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inTrOducTiOn The production of human monoclonal mAbs for research and clinical use is closely related to the development of phage display technology, initially described by Smith in 1985 and further developed by other groups (e.g., Winter, McCafferty, Lerner, Barbas). Antibody phage display (APD) is based on genetic engineering of bacteriophages (viruses that infect bacteria) and repeated round...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00294bm12